Coding

Part:BBa_K2570022

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-10-05)


araC＋mazf+GFP

This is an improved version of BBa_K1405008. MazF is a sequence-specific mRNA endoribonuclease that initiates a programmed cell death pathway in response to various stresses. The expression product of MazF is a stable toxin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2244
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None